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  • Article libreArticleEn anglais

    Anthropometric characteristics between ever and never screened for hypertension in Burkina Faso

    Jeoffray Diendéré, Toussaint Rouamba, Jean Kaboré, Augustin N. Zeba, Halidou Tinto, Sylvin Ouédraogo, Athanase Millogo, Séni Kouanda

    BackgroundExcess body weight was associated with a higher chance for hypertension detection.AimTo compare the anthropometric characteristics and blood pressure levels between Burkinabè adults who had ever been screened for hypertension and those who had never been screened, and to assess the associated factors with the uptake of hypertension screening.SettingUrban and rural Burkina Faso.MethodsThis was a secondary analysis using the Burkina Faso 2013 WHO Stepwise approach to Surveillance cross-sectional survey. Data from 3831 adult men and women were analysed. Descriptive and analytical analyses were performed using Student’s t, ANOVA, χ2, Fisher’s exact tests and logistic regression.ResultsAmong participants, 41.6% (95% CI: 40.0–43.1) had never been screened for hypertension, and compared to those who had ever been screened, they had significantly lower mean weight, waist circumference and body mass index, and lower prevalence of overweight or obesity and abdominal obesity. The prevalence of prehypertension was similar between the two groups (around 40%) and the prevalence of hypertension was lower in those who had never been screened (17.3% vs 20.8%; p = 0.007). Overweight or obesity (adjusted odds ratio [aOR] = 1.3; p = 0.03) and abdominal obesity (aOR = 1.3; p = 0.002) were associated with screening uptake.ConclusionThe Burkinabè adults who had never been screened for hypertension were apparently thin, but pre-hypertension or hypertension was also common among them. Increasing body size (excess weight or abdominal obesity) may be the reason for screening uptake.ContributionSpecific awareness-raising messages to motivate slim people to undergo screening need to be developed.

    Biostatistiques et épidémiologieMédecine interne (cardiologie, endocrinologie, néphrologie…)Santé publiqueLicence : Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.
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  • Article libreArticleEn anglais

    Anthropometric Differences in Community- Versus Clinic-Recruited Infants Participating in a Trial of Azithromycin for Prevention of Childhood Mortality in Burkina Faso

    Mamadou Ouattara, Ali Sié, Mamadou Bountogo, Valentin Boudo, Clarisse Dah, Elodie Lebas, Huiyu Hu, Travis C. Porco, Benjamin F. Arnold, Thomas M. Lietman, Catherine E. Oldenburg

    ABSTRACT.Clinic-based recruitment for preventative interventions for child health may select for healthier populations compared with community-based outreach. Nutritional status during infancy as measured by anthropometry is predictive of mortality, growth faltering later in life, and poor cognitive development outcomes. We evaluated baseline differences in infant nutritional status among children recruited directly in their community versus clinic recruitment among infants participating in a trial of azithromycin compared with placebo for prevention of mortality in three districts of Burkina Faso. Infants between 5 and 12 weeks of age were recruited in their community of residence via vaccine outreach teams or in primary health-care clinics during vaccine clinics. Weight, height, and mid upper arm circumference were measured. We used linear and logistic regression models to compare anthropometric outcomes among community and clinic recruited infants, adjusting for age at enrollment, gender, and season. Among 32,877 infants enrolled in the trial, 21,273 (64.7%) were recruited via community outreach. Mean weight-for-age z-score (WAZ) was −0.60 ± 1.2 (SD), weight-for-length z-score (WLZ) was −0.16 ± 1.5, and length-for-age z-score was−0.53 ± 1.3. Infants enrolled in the community had lower WAZ (mean difference, −0.12; 95% CI, −0.20 to −0.04) and WLZ (mean difference, −0.21; 95% CI, −0.32 to −0.09). Community-recruited infants were more often underweight (WAZ < −2; odds ratio [OR], 1.25; 95% CI, 1.09–1.43) and wasted (WLZ < −2; OR, 1.54; 95% CI, 1.31–1.79). There was no evidence of a difference in height-based measures. Community and clinic recruitment likely reach different populations of children.

    ImmunologiePédiatrieSanté publiqueLicence : Creative Commons Attribution (CC-BY) License This is an open-access article distributed under the terms of the, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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  • Article libreArticleEn anglais

    Anthropometry at birth and at age of routine vaccination to predict mortality in the first year of life: A birth cohort study in BukinaFaso

    BackgroundThe World Health Organization currently defines severe acute malnutrition (SAM) in infants aged under 6 months of age using weight-for-length Z score (WLZ). Given widespread use of mid-upper arm circumference (MUAC) for identifying SAM in older children and weight-for-age (WAZ) for growth monitoring, there is increasing debate about the optimal anthropometric criteria to best identify infants u6m at-risk of mortality.ObjectiveTo determine the discriminatory value for mortality during the first 12 months of life of anthropometry taken at birth and at age two months (approximate age of routine vaccination).DesignData were analyzed from a birth cohort recruited between April and December of 2004 at four health facilities within Bansalogho District in Burkina Faso. Infants were followed up for 12 months. Mortality risks were estimated using hazards ratios (HR). Discriminatory value was assessed using receiver operating characteristic curves.ResultsOf 1,103 infants, 227 (21%) were low birthweight (LBW). During 12 months, 86 (7.8%) infants died; 38 (44%) among the LBW group. At birth, MUAC<9.0cm, weight<2.5kg, length<44.2cm and incalculable WLZ were associated with mortality. Sixty (70%) deaths occurred after the age of two months; 26 (43%) among LBW infants. At age two months, any MUAC <11.5cm, weight <3.8kg (WAZ<-3) and length <52.4cm (LAZ<-3) were associated with risk of mortality. WLZ was not associated with mortality at any threshold.Birth weight did not modify the effect of the association between month two MUAC and one-year mortality (P = 0.33).ConclusionInfants at heightened risk of mortality and are better identified during early infancy by MUAC or WFA than by WLZ. LBW infants with low anthropometry at the age of routine immunizations remain at elevated risk than normal birth weight (NBW)infants and require intervention. Effectiveness, cost-effectiveness and coverage of applying proposed thresholds should be investigated as a priority to inform policy a

    Biostatistiques et épidémiologieImmunologiePédiatrieLicence : Creative Commons Attribution License This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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  • Article libreArticleEn anglais

    Anti-HCV Tannins From Plants Traditionally Used in West Africa and Extracted With Green Solvents

    Moussa Bamba, Simon Bordage, Marie-Emmanuelle Sahuc, Sophie Moureu, Jennifer Samaillie, Vincent Roumy, Peggy Vauchel, Krasimir Dimitrov, Yves Rouillé, Jean Dubuisson, Fézan Honora Tra Bi, Karin Séron, Sevser Sahpaz

    Millions of people are still infected with hepatitis C virus (HCV) nowadays. Although recent antivirals targeting HCV proteins are very efficient, they are not affordable for many people infected with this virus. Therefore, new and more accessible treatments are needed. Several Ivorian medicinal plants are traditionally used to treat “yellow malaria”, a nosological category including illness characterized by symptomatic jaundice such as hepatitis. Therefore, some of these plants might be active against HCV. An ethnobotanical survey in Côte d’Ivoire allowed us to select such medicinal plants. Those were first extracted with methanol and tested for their anti-HCV activity. The most active ones were further studied to specify their IC50 and to evaluate their toxicity in vitro. Greener solvents were tested to obtain extracts with similar activities. Following a phytochemical screening, tannins of the most active plants were removed before re-testing on HCV. Some of these tannins were identified by UPLC-MS and pure molecules were tested against HCV. Out of the fifteen Ivorian medicinal plants selected for their putative antiviral activities, Carapa procera DC. and Pericopsis laxiflora (Benth. ex Baker) Meeuwen were the most active against HCV (IC50: 0.71 and 0.23 μg/ml respectively) and not toxic for hepatic cells. Their crude extracts were rich in polyphenols, including tannins such as procyanidins A2 which is active against HCV. The same extracts without tannin lost their anti-HCV activity. Replacing methanol by hydro-ethanolic solvent led to tannins-rich extracts with similar antiviral activities, and higher than that of aqueous extracts.

    Biostatistiques et épidémiologieParasitologie et mycologiePharmacognosie, phytothérapie et médecine traditionnelleLicence : This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journa
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  • Article libreArticleEn anglais

    Anti-Inflammatory and Anti-Colon Cancer Activities of Mung Bean Grown in Burkina Faso

    Wendmintiri Jeanne d'Arc Kabré, Durand Dah-Nouvlessounon, Fatoumata/Ba Hama, N. Arnaud Kohonou, Haziz Sina, Maximin Senou, Lamine Baba-Moussa, Aly Savadogo

    Widely used in traditional medicine in Asia and recently introduced in Burkina Faso under the name Beng-tigré, mung bean is a legume consumed throughout the world and more so in India. The objective of this study was to evaluate the cytotoxicity of the mung bean grown and consumed in Burkina Faso and to study its biological properties such as anti-inflammatory and anticancer activity of the natural and sprouted seeds. The cytotoxicity of the extracts was tested on Artemia salina larvae, and the anti-inflammatory activity was evaluated in vitro by albumin denaturation method using diclofenac as reference molecule. The anticancer activity of hydro-ethanol extracts was evaluated on rats made cancerous with 1,2-dimethylhydrazine (DMH) using 5-fluorouracil as reference molecule. The results showed that the highest yield of the plant extraction was observed with the hydro-ethanol solvent, both for the natural form of mung bean (MBN) and for its sprouted form (MBG). The cytotoxicity test showed no toxicity of the extracts toward shrimp larvae. The ethanolic extract of germinated mung bean seeds gave the highest anti-inflammatory activity at 95.13 ± 0.22% inhibition with significant difference (p < 0.05) between the extracts. Cancer induction with DMH was inhibited by both MBN and MBG extracts. The test of preventive effects of the extracts showed the best activity with significant difference in biochemical results. These results confirm that the mung bean grown in Burkina Faso, as a nontoxic legume, is a functional food that can be integrated into the population's dietary habits for a double interest. Moreover, they open perspectives for the research of active principles of plant origin with anti-inflammatory and anticancer properties.

    Santé publiqueLicence : This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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  • Article libreArticleEn anglais

    Anti-inflammatory effects of perioperative statin therapy

    Wilton A. van Klei, Wolfgang F. Buhre

    Licence : This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
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  • Article libreArticleEn anglais

    Anti-malarial efficacy and resistance monitoring of artemether-lumefantrine and dihydroartemisinin-piperaquine shows inadequate efficacy in children in Burkina Faso, 2017–2018

    Adama Gansané, Leah F. Moriarty, Didier Ménard, Isidore Yerbanga, Esperance Ouedraogo, Paul Sondo, Rene Kinda, Casimir Tarama, Edwige Soulama, Madou Tapsoba, David Kangoye, Cheick Said Compaore, Ousmane Badolo, Blami Dao, Samuel Tchwenko, Halidou Tinto, Innocent Valea

    BackgroundThe World Health Organization recommends regularly assessing the efficacy of artemisinin-based combination therapy (ACT), which is a critical tool in the fight against malaria. This study evaluated the efficacy of two artemisinin-based combinations recommended to treat uncomplicated Plasmodium falciparum malaria in Burkina Faso in three sites: Niangoloko, Nanoro, and Gourcy.MethodsThis was a two-arm randomized control trial of the efficacy of artemether-lumefantrine (AL) and dihydroartemisinin-piperaquine (DP). Children aged 6–59 months old were monitored for 42 days. The primary outcomes of the study were uncorrected and PCR-corrected efficacies to day 28 for AL and 42 for DP. Molecular markers of resistance to artemisinin derivatives and partner drugs were also analysed.ResultsOf 720 children enrolled, 672 reached study endpoints at day 28, 333 in the AL arm and 339 in the DP arm. PCR-corrected 28-day per protocol efficacy in the AL arm was 74% (64–83%) in Nanoro, 76% (66–83%) in Gourcy, and 92% (84–96%) in Niangoloko. The PCR-corrected 42-day per protocol efficacy in the DP arm was 84% (75–89%) in Gourcy, 89% (81–94%) in Nanoro, and 97% (92–99%) in Niangoloko.No Pfk13 mutation previously associated with artemisinin-resistance was observed. No statistically significant association was found between treatment outcome and presence of the 86Y mutation in the Pfmdr1 gene. There was also no association observed between treatment outcome and Pfpm2 or Pfmdr1 copy number variation.ConclusionThe results of this study indicate evidence of inadequate efficacy of AL at day 28 and DP at day 42 in the same two sites. A change of first-line ACT may be warranted in Burkina Faso.Trial Registry Pan African Clinical Trial Registry Identifier: PACTR201708002499311.Date of registration: 8/3/2017https://pactr.samrc.ac.za/Search.aspx

    Biologie cellulaire et moléculaireGénétiqueMicrobiologie (bactériologie, virologie)Licence : Open Access http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/publicdomain/zero/1.0/ This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as
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  • Article libreArticleEn anglais

    Anti-nociceptive properties in rodents and the possibility of using polyphenol-rich fractions from sida urens L. (Malvaceae) against of dental caries bacteria

    Kiessoun Konaté, Patrice Zerbo, Maurice Ouédraogo, Crépin I Dibala, Hilou Adama, Oksana Sytar, Marian Brestic, Nicolas Barro

    BackgroundSida urens L. (Malvaceae) is in flora of Asian medicinal herbs and used traditionally in West of Burkina Faso for the treatment of infectious diseases and particularly used against, dental caries bacteria, fever, pain and possesses analgesic properties. This study was conducted to reveal the antibacterial effect against dental caries bacteria on the one hand, and evaluate their analgesic capacity in experimental model with Swiss mice and on the other hand, with an aim to provide a scientific basis for the traditional use of this plant for the management of dental caries bacteria.MethodThe antibacterial assays in this study were performed by using inhibition zone diameters, MIC (Minimum inhibitory concentration) and MBC (Minimal bactericidal concentration) methods. On the whole the dental caries bacteria (Gram-positive and Gram-negative bacterial strains) were used. Negative control was prepared using discs impregnated with 10% DMSO in water and commercially available Gentamicin from Alkom Laboratories LTD was used as positive reference standards for all bacterial strains. In acute toxicity test, mice received doses of extract (acetone/water extract) from Sida urens L. by intraperitoneal route and LD50 was determined in Swiss mice. As for analgesic effects, acetic acid writhing method was used in mice. The acetic acid-induced writhing method was used in mice with aim to study analgesic effects.ResultsThe results showed that the highest antibacterial activities were founded with the polyphenol-rich fractions against all bacterial strains compared to the standard antibiotic. About preliminary study in acute toxicity test, LD50 value obtained was more than 5000 mg/kg b.w. Polyphenol-rich fractions produced significant analgesic effects in acetic acid-induced writhing method and in a dose-dependent inhibition was observed.ConclusionThese results validate the ethno-botanical use of Sida urens L. (Malvaceae) and demonstrate the potential of this herbaceous as a p

    Microbiologie (bactériologie, virologie)ImmunologiePharmacologieLicence : http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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  • Article libreArticleEn anglais

    Anti-plasmodial activity of Dicoma tomentosa (Asteraceae) and identification of urospermal A-15-O-acetate as the main active compound

    Olivia Jansen, Monique Tits, Luc Angenot, Jean-Pierre Nicolas, Patrick De Mol, Jean-Baptiste Nikiema, Michel Frédérich

    BackgroundNatural products could play an important role in the challenge to discover new anti-malarial drugs. In a previous study, Dicoma tomentosa (Asteraceae) was selected for its promising anti-plasmodial activity after a preliminary screening of several plants traditionally used in Burkina Faso to treat malaria. The aim of the present study was to further investigate the anti-plasmodial properties of this plant and to isolate the active anti-plasmodial compounds.MethodsEight crude extracts obtained from D. tomentosa whole plant were tested in vitro against two Plasmodium falciparum strains (3D7 and W2) using the p-LDH assay (colorimetric method). The Peters’ four-days suppressive test model (Plasmodium berghei-infected mice) was used to evaluate the in vivo anti-plasmodial activity. An in vitro bioguided fractionation was undertaken on a dichloromethane extract, using preparative HPLC and TLC techniques. The identity of the pure compound was assessed using UV, MS and NMR spectroscopic analysis. In vitro cytotoxicity against WI38 human fibroblasts (WST-1 assay) and haemolytic activity were also evaluated for extracts and pure compounds in order to check selectivity.ResultsThe best in vitro anti-plasmodial results were obtained with the dichloromethane, diethylether, ethylacetate and methanol extracts, which exhibited a high activity (IC50 ≤ 5 μg/ml). Hot water and hydroethanolic extracts also showed a good activity (IC50 ≤ 15 μg/ml), which confirmed the traditional use and the promising anti-malarial potential of the plant. The activity was also confirmed in vivo for all tested extracts. However, most of the active extracts also exhibited cytotoxic activity, but no extract was found to display any haemolytic activity. The bioguided fractionation process allowed to isolate and identify a sesquiterpene lactone (urospermal A-15-O-acetate) as the major anti-plasmodial compound of the plant (IC50 < 1 μg/ml against both 3D7 and W2 strains). This was also found to be th

    Parasitologie et mycologiePharmacologieMaladies infectieuses et tropicalesLicence : http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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  • Article libreArticleEn anglais

    Antibacterial activity against β- lactamase producing Methicillin and Ampicillin-resistants Staphylococcus aureus: fractional Inhibitory Concentration Index (FICI) determination

    Kiessoun Konaté, Jacques François Mavoungou, Alexis Nicaise Lepengué, Raïssa RR Aworet-Samseny, Adama Hilou, Alain Souza, Mamoudou H Dicko, Bertrand M’Batchi

    BackgroundThe present study reports the antibacterial capacity of alkaloid compounds in combination with Methicillin and Ampicillin-resistants bacteria isolated from clinical samples. The resistance of different bacteria strains to the current antibacterial agents, their toxicity and the cost of the treatment have led to the development of natural products against the bacteria resistant infections when applied in combination with conventional antimicrobial drugs.MethodThe antibacterial assays in this study were performed by using inhibition zone diameters, MIC, MBC methods, the time-kill assay and the Fractional Inhibitory Concentration Index (FICI) determination. On the whole, fifteen Gram-positive bacterial strains (MRSA/ARSA) were used. Negative control was prepared using discs impregnated with 10 % DMSO in water and commercially available Methicillin and Ampicillin from Alkom Laboratories LTD were used as positive reference standards for all bacterial strains.ResultsWe noticed that the highest activities were founded with the combination of alkaloid compounds and conventional antibiotics against all bacteria strains. Then, results showed that after 7 h exposition there was no viable microorganism in the initial inoculums.ConclusionThe results of this study showed that alkaloid compounds in combination with conventional antibiotics (Methicillin, Ampicillin) exhibited antimicrobial effects against microorganisms tested. These results validate the ethno-botanical use of Cienfuegosia digitata Cav. (Malvaceae) in Burkina Faso. Moreover, this study demonstrates the potential of this herbaceous as a source of antibacterial agent that could be effectively used for future health care purposes.

    Microbiologie (bactériologie, virologie)PharmacologieSanté publiqueLicence : http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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  • Article libreArticle

    Antibio-résistance des souches de Salmonella spp isolées d'hémocultures à Bukavu en RD Congo

    Théophile Mitima Kashosi, Archippe Birindwa Muhandule, David Lupande Mwenebitu, Nicolas Mihuhi, John Kivukuto Mutendela, Kanigula Mubagwa

    IntroductionLa fièvre typhoïde est un problème majeur de santé publique dans les pays en voie de développement jusqu'à ce jour à cause de la vétusté des infrastructures sanitaires et des circuits de distribution de l'eau presque inexistants. En RDC en général et à Bukavu en particulier, l'hémoculture est inaccessible à la majorité des patients. L'objectif de cette étude était d'évaluer la sensibilité de Salmonella spp aux antibiotiques couramment utilisés dans la prise en charge de la fièvre typhoïde à Bukavu.MéthodesUne étude transversale étalée sur 6 mois a était organisée. Tout malade suspect de la fièvre typhoïde a été sélectionné dans l'étude. L'hémoculture était faite systématiquement chez tout malade sélectionné. L'identification de la souche bactérienne et l'antibiogramme ont été réalisés par les méthodes conventionnelles. Les antibiotiques suivants ont été testés: amikacine, amoxicilline, augmentin, aeftazidime, ceftriaxone, cefuroxime, chloramphénicol, ciprofloxacine, cotrimoxazole, doxycycline, gentamicine, négram, norfloxacine.Résultats460 malades ont été sélectionnés dans l'étude. 144 (31,30%) hémocultures positives ont été observées. Salmonella spp était le germe le plus isolé (41,66%). Les souches de Salmonella spp isolées à Bukavu sont sensibles à la ciprofloxacine (91,7%), au ceftazidime (81,7%), ceftriaxone (80%), norfloxacine (80%), amikacine (76,6%) et au cefuroxime (73,3%). Elles restent résistantes aux autres molécules d'antibiotiques.ConclusionCes résultats montrent une sensibilité diminuée à plupart des antibiotiques testés. Un test d'antibiogramme est requis en cas de fièvre typhoïde pour une meilleure prise en charge.

    Microbiologie (bactériologie, virologie)PharmacologieLicence : The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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  • Article libreArticle

    Antibioprophylaxie dans les chirurgies gynécologiques et obstétricales propres et propres contaminées à l'Hôpital Général de Yaoundé, Cameroun

    Jean Dupont Kemfang Ngowa, Anny Ngassam, R Motzebo Mbouopda, Jean Marie Kasia

    IntroductionIl s'agira de déterminer l'incidence et les facteurs associés à l'infection post opératoire dans les chirurgies gynécologiques et obstétricales propres et propres contaminées.MéthodesÉtude de cohorte prospective axée sur la surveillance de l'infection post opératoire chez les patientes opérées d'une chirurgie gynécologique ou obstétricale propre ou propre contaminée au Service de Gynécologie B de l'Hôpital Général de Yaoundé, après une antibioprophylaxie avec la cefazoline. La période d’étude de 18 mois s'est étalée de janvier 2012 à juin 2013. La surveillance des patientes opérées se poursuivait sur les 30 premiers jours post opératoires.RésultatsAu total 211 patientes opérées d'une chirurgie gynécologique ou obstétricale propre ou propre contaminée ont été enrôlées dans cette étude. La moyenne d’âge des patientes était de 35,4 ±10 ans; les chirurgies propres contaminées représentaient 76,3% et les chirurgies propres 23,7%. Nous avons observé au total 06 (2,8%) cas d'infection post opératoire, dont 03 (1,4%) cas d'infection du site opératoire, 02 (0,95%) cas d'infection urinaire et 01(0,47%) cas d'infection pulmonaire. Les variables significativement associées à l'infection post opératoire étaient la durée de la chirurgie (durée moyenne: 126± 89min vs 82± 50 min; p< 0,04) et le diabète (OR. 40,8; IC à 95%. 2,2-749,2; p < 0,000.ConclusionNotre taux d'infection post opératoire global de 2,8% et d'infection du site opératoire de 1,4% vient renforcer les données de la littérature sur l'efficacité de l'antibioprophylaxie dans les chirurgies gynécologiques et obstétricales propres et propres contaminées en milieu africain. Cependant le respect des principes d'asepsie péri opératoire et une sélection précise de la classe de chirurgie devraient être de règle dans notre contexte.

    Biostatistiques et épidémiologieGynécologie-obstétriqueSanté publiqueLicence : The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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    Antibiothérapie probabiliste des pneumopathies communautaires graves de l'adulte immunocompétent : étude rétrospective

    Anne-Laure Colasse

    Introduction : en France, les pneumopathies aiguë communautaires graves (PACG) sont responsable de 30 % des décès annuels en réanimation. L'antibiothérapie initiée aux urgences est essentielle pour améliorer les résultats cliniques. L'objectif principal était d'évaluer la qualité des prescriptions d'antibiotiques au urgences vitales (SAUV) pour les PACG. Matériel et méthode : étude rétrospective, CHU Amiens du 1er janvier 2022 au 31 décembre 2023, portant sur les prescriptions d'antibiothérapie probabiliste pour les PACG chez les patients admis au SAUV. Résultats : 188 prescriptions ont été analysées, 58% des hommes, avec une moyenne d'âge de 66 ans et 84% des patients avaient une autonomie GIR 6. Les traitements comprenaient une mono-antibiothérapie (36%) et une bi-antibiothérapie (41%). 38 % des prescriptions étaient conformes aux recommandations. Les principales causes de non-conformité incluaient l'absence d'antibiothérapie (18%) et l'utilisation inadaptée en termes de molécule et de posologie (28%). Parmi les germes identifiés (53% des cas), les principaux étaient le COVID-19 (18%) et le pneumocoque (14%). La VNI a été associée à une meilleure conformité des prescriptions d'antibiothérapie (50% vs. 31%). Conclusion : un tiers des prescriptions d'antibiothérapie étaient conformes aux recommandations. Les principaux germes identifiés étaient le COVID-19 et le pneumocoque. Il est impératif d'améliorer les pratiques de prescription pour aider les cliniciens à choisir l'antibiothérapie adéquate.

    Microbiologie (bactériologie, virologie)ImmunologiePharmacologieLicence : https://creativecommons.org/licenses/by/4.0/
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  • Article libreArticleEn anglais

    Antibiotic Susceptibility of Staphylococcus aureus and Streptococcus pneumoniae Isolates from the Nasopharynx of Febrile Children under 5 Years in Nanoro, Burkina Faso

    (1) Background: nasopharynx colonization by resistant Staphylococcus aureus and Streptococcus pneumoniae can lead to serious diseases. Emerging resistance to antibiotics commonly used to treat infections due to these pathogens poses a serious threat to the health system. The present study aimed to determine the antibiotic susceptibility of S. aureus and S. pneumoniae isolates from the febrile children’s nasopharynx under 5 years in Nanoro (Burkina Faso). (2) Methods: bacterial isolates were identified from nasopharyngeal swabs prospectively collected from 629 febrile children. Antibiotic susceptibility of S. aureus and S. pneumoniae isolates was assessed by Kirby–Bauer method and results were interpreted according to the Clinical and Laboratory Standard Institute guidelines. (3) Results: bacterial colonization was confirmed in 154 (24.5%) of children of whom 96.1% carried S. aureus, 3.2% had S. pneumoniae, and 0.6% carried both bacteria. S. aureus isolates showed alarming resistance to penicillin (96.0%) and S. pneumoniae was highly resistant to tetracycline (100%) and trimethoprim–sulfamethoxazole (83.3%), and moderately resistant to penicillin (50.0%). Furthermore, 4.0% of S. aureus identified were methicillin resistant. (4) Conclusion: this study showed concerning resistance rates to antibiotics to treat suspected bacterial respiratory tract infections. The work highlights the necessity to implement continuous antibiotic resistance surveillance.

    Microbiologie (bactériologie, virologie)PharmacologiePédiatrieLicence : https://creativecommons.org/licenses/by/4.0/ Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ().
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    Antibiotiques en agriculture : réduire leur usage tout en limitant les risques sanitaires et socioéconomiques au Sud

    François Roger, Christian Ducrot

    L’usage massif des antibiotiques en agriculture augmente avec l’essor des élevages intensifs et de la demande en produits animaux. Un phénomène grave en résulte, l’antibiorésistance : des bactéries développent des mécanismes de résistance aux antibiotiques, résistance qui se diffuse dans les populations bactériennes, y compris celles affectant l’homme. L’antibiorésistance a un impact majeur sur la santé publique, et ses effets sur la santé animale et la biodiversité sont encore mal connus. Face à ce fléau, réduire et rationaliser l’usage des antibiotiques est une urgence mondiale, au Nord comme au Sud. Cela requiert des actions intersectorielles impliquant les professionnels et les chercheurs dans de multiples domaines des sciences : les animaux domestiques, la faune sauvage, les plantes, l’homme et l’environnement. Ces actions sont particulièrement complexes à mettre en œuvre dans les pays du Sud.

    Microbiologie (bactériologie, virologie)PharmacologiePharmacognosie, phytothérapie et médecine traditionnelleLicence : https://creativecommons.org/licenses/by/4.0/
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  • Article libreArticleEn anglais

    Antibodies to malaria vaccine candidates are associated with chloroquine or sulphadoxine/pyrimethamine treatment efficacy in children in an endemic area of Burkina Faso

    Amidou Diarra, Issa Nebie, Alfred Tiono, Issiaka Soulama, Alphonse Ouedraogo, Amadou Konate, Michael Theisen, Daniel Dodoo, Alfred Traore, Sodiomon B Sirima

    BackgroundPatient immune status is thought to affect the efficacy of anti-malarial chemotherapy. This is a subject of some importance, since evidence of immunity-related interactions may influence our use of chemotherapy in populations with drug resistance, as well as assessment of the value of suboptimal vaccines. The study aim was to investigate relationship between antibodies and anti-malarial drug treatment outcomes.MethodsSome 248 children aged 0.5 and 15 years were recruited prior to the high malaria transmission season. Venous blood (5 ml) was obtained from each child to measure antibody levels to selected malaria antigens, using ELISA. Blood smears were also performed to assess drug efficacy and malaria infection prevalence. Children were actively followed up to record clinical malaria cases.ResultsIgG levels to MSP3 were always higher in the successfully treated group than in the group with treatment failure. The same observation was made for GLURP but the reverse observation was noticed for MSP1-19. Cytophilic and non-cytophilic antibodies were significantly associated with protection against all three antigens, except for IgG4 to MSP1-19 and GLURP.ConclusionAcquired anti-malarial antibodies may play an important role in the efficacy of anti-malarial drugs in younger children more susceptible to the disease.

    Biostatistiques et épidémiologieMicrobiologie (bactériologie, virologie)Parasitologie et mycologieLicence : http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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  • Article libreArticleEn anglais

    Antibody levels against GLURP R2, MSP1 block 2 hybrid and AS202.11 and the risk of malaria in children living in hyperendemic (Burkina Faso) and hypo-endemic (Ghana) areas

    Bright Adu, Mariama K. Cherif, Samuel Bosomprah, Amidou Diarra, Fareed K. N. Arthur, Emmanuel K. Dickson, Giampietro Corradin, David R. Cavanagh, Michael Theisen, Sodiomon B. Sirima, Issa Nebie, Daniel Dodoo

    Background Differences in parasite transmission intensity influence the process of acquisition of host immunity to Plasmodium falciparum malaria and ultimately, the rate of malaria related morbidity and mortality. Potential vaccines being designed to complement current intervention efforts therefore need to be evaluated against different malaria endemicity backgrounds.MethodsThe associations between antibody responses to the chimeric merozoite surface protein 1 block 2 hybrid (MSP1 hybrid), glutamate-rich protein region 2 (GLURP R2) and the peptide AS202.11, and the risk of malaria were assessed in children living in malaria hyperendemic (Burkina Faso, n = 354) and hypo-endemic (Ghana, n = 209) areas. Using the same reagent lots and standardized protocols for both study sites, immunoglobulin (Ig) M, IgG and IgG sub-class levels to each antigen were measured by ELISA in plasma from the children (aged 6–72 months). Associations between antibody levels and risk of malaria were assessed using Cox regression models adjusting for covariates.ResultsThere was a significant association between GLURP R2 IgG3 and reduced risk of malaria after adjusting age of children in both the Burkinabe (hazard ratio 0.82; 95 % CI 0.74–0.91, p < 0.0001) and the Ghanaian (HR 0.48; 95 % CI 0.25–0.91, p = 0.02) cohorts. MSP1 hybrid IgM was associated (HR 0.85; 95 % CI 0.73–0.98, p = 0.02) with reduced risk of malaria in Burkina Faso cohort while IgG against AS202.11 in the Ghanaian children was associated with increased risk of malaria (HR 1.29; 95 % CI 1.01–1.65, p = 0.04).ConclusionThese findings support further development of GLURP R2 and MSP1 block 2 hybrid, perhaps as a fusion vaccine antigen targeting malaria blood stage that can be deployed in areas of varying transmission intensity.Electronic supplementary materialThe online version of this article (doi:10.1186/s12936-016-1146-4) contains supplementary material, which is available to authorized users.

    Biostatistiques et épidémiologieParasitologie et mycologieImmunologieLicence : Open Access http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/publicdomain/zero/1.0/ This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (), which permits unrestricted use, distribution, and reproduction in any medium, provid
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  • Article libreArticleEn anglais

    Antibody responses to P. falciparum blood stage antigens and incidence of clinical malaria in children living in endemic area in Burkina Faso

    Mariama K. Cherif, Oumarou Ouédraogo, Guillaume S. Sanou, Amidou Diarra, Alphonse Ouédraogo, Alfred Tiono, David R. Cavanagh, Theisen Michael, Amadou T. Konaté, Nora L. Watson, Megan Sanza, Tina J. T. Dube, Sodiomon B. Sirima, Issa Nebié

    BackgroundHigh parasite-specific antibody levels are generally associated with low susceptibility to Plasmodium falciparum malaria. This has been supported by several studies in which clinical malaria cases of P. falciparum malaria were reported to be associated with low antibody avidities. This study was conducted to evaluate the role of age, malaria transmission intensity and incidence of clinical malaria in the induction of protective humoral immune response against P. falciparum malaria in children living in Burkina Faso.MethodsWe combined levels of IgG and IgG subclasses responses to P. falciparum antigens: Merozoite Surface Protein 3 (MSP3), Merozoite Surface Protein 2a (MSP2a), Merozoite Surface Protein 2b (MSP2b), Glutamate Rich Protein R0 (GLURP R0) and Glutamate Rich Protein R2 (GLURP R2) in plasma samples from 325 children under five (05) years with age, malaria transmission season and malaria incidence.ResultsWe notice higher prevalence of P. falciparum infection in low transmission season compared to high malaria transmission season. While, parasite density was lower in low transmission than high transmission season. IgG against all antigens investigated increased with age. High levels of IgG and IgG subclasses to all tested antigens except for GLURP R2 were associated with the intensity of malaria transmission. IgG to MSP3, MSP2b, GLURP R2 and GLURP R0 were associated with low incidence of malaria. All IgG subclasses were associated with low incidence of P. falciparum malaria, but these associations were stronger for cytophilic IgGs.ConclusionsOn the basis of the data presented in this study, we conclude that the induction of humoral immune response to tested malaria antigens is related to age, transmission season level and incidence of clinical malaria.

    Biostatistiques et épidémiologieParasitologie et mycologieImmunologieLicence : Open Access http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/publicdomain/zero/1.0/ This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (), which permits unrestricted use, distribution, and reproduction in any medium, provid
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  • Article libreArticleEn anglais

    Antibody responses to α-Gal in African children vary with age and site and are associated with malaria protection

    Ruth Aguilar, Itziar Ubillos, Marta Vidal, Núria Balanza, Núria Crespo, Alfons Jiménez, Augusto Nhabomba, Chenjerai Jairoce, David Dosoo, Ben Gyan, Aintzane Ayestaran, Hèctor Sanz, Joseph J. Campo, Gloria P. Gómez-Pérez, Luis Izquierdo, Carlota Dobaño

    Naturally-acquired antibody responses to malaria parasites are not only directed to protein antigens but also to carbohydrates on the surface of Plasmodium protozoa. Immunoglobulin M responses to α-galactose (α-Gal) (Galα1-3Galβ1-4GlcNAc-R)-containing glycoconjugates have been associated with protection from P. falciparum infection and, as a result, these molecules are under consideration as vaccine targets; however there are limited field studies in endemic populations. We assessed a wide breadth of isotype and subclass antibody response to α-Gal in children from Mozambique (South East Africa) and Ghana (West Africa) by quantitative suspension array technology. We showed that anti-α-Gal IgM, IgG and IgG1–4 levels vary mainly depending on the age of the child, and also differ in magnitude in the two sites. At an individual level, the intensity of malaria exposure to P. falciparum and maternally-transferred antibodies affected the magnitude of α-Gal responses. There was evidence for a possible protective role of anti-α-Gal IgG3 and IgG4 antibodies. However, the most consistent findings were that the magnitude of IgM responses to α-Gal was associated with protection against clinical malaria over a one-year follow up period, especially in the first months of life, while IgG levels correlated with malaria risk.

    Parasitologie et mycologieImmunologieMaladies infectieuses et tropicalesLicence : Open Access http://creativecommons.org/licenses/by/4.0/ This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original autho
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    Anticoagulants oraux directs et fonction rénale : rôle du pharmacien d’officine dans la prise en charge des patients

    Rémnaka Anakoma

    Cette thèse explore l'utilisation des anticoagulants oraux directs (AOD) chez les patients âgés. Elle met l'accent sur l'importance du suivi de la fonction rénale. L'étude a été menée auprès de 35 patients d'âge moyen 77,4 ans. Elle révèle des lacunes significatives dans la connaissance des patients concernant leur traitement et la surveillance rénale nécessaire. Les résultats montrent que seulement 34,3 % des patients savent que leur traitement AOD doit être ajusté à la fonction rénale ; 48,6 % sont informés de la nécessité d'un contrôle annuel de leur fonction rénale et 28,6 % des patients ont déjà nécessité un ajustement posologique principalement en raison d'une insuffisance rénale. Ils montrent également que 85,7 % des patients sont favorables au partage d'informations entre le laboratoire d'analyses et la pharmacie. L'étude souligne l'importance cruciale de l'éducation thérapeutique des patients sous AOD particulièrement concernant le suivi de la fonction rénale. Le pharmacien d'officine, par le biais d'entretiens pharmaceutiques, peut jouer un rôle clé pour sensibiliser les patients à ce propos et prévenir les complications iatrogéniques. Cependant, des freins structurels et organisationnels limitent encore l'efficacité de ces dispositifs d'accompagnement. Les résultats ouvrent des perspectives pour une meilleure coordination des soins et soulignent la nécessité d'optimiser les outils d'accompagnement des patients. Des recherches futures pourraient approfondir ces résultats sur un échantillon plus large et explorer l'impact à long terme des programmes d'éducation thérapeutique sur la sécurité et l'efficacité des traitements par AOD. Enfin, elle ouvre la voie à une réflexion plus élargie sur la prise en charge des médicaments à risque d'insuffisance rénale, en vue d'une optimisation de la pharmacothérapie pour les patients âgés et polymédicamentés.

    PharmacologieToxicologiePharmacie galéniqueLicence : https://creativecommons.org/licenses/by/4.0/
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    Anticorps anti-CD38 dans le myélome multiple

    Jérôme Moreaux

    La forte expression de la molécule CD38 par les cellules plasmocytaires ainsi que son rôle biologique dans la régulation de l’adhérence et la migration cellulaire, avec des fonctions de signalisation, a conduit au développement d’anticorps spécifiques pour le traitement de patients atteints de myélome multiple (MM). Ces anticorps induisent en effet la mort des cellules de myélome multiple par des mécanismes de lyse cellulaire dépendante du complément (CDC), de cytotoxicité cellulaire dépendante des anticorps (ADCC), de phagocytose cellulaire dépendant des anticorps (ADCP), mais aussi par des mécanismes directs d’induction de mort cellulaire. Ils ont de plus des effets immunomodulateurs liés à l’élimination de cellules immunitaires immunosuppressives qui expriment également CD38. Bien qu’ayant des actions variables par rapport à ce registre d’activité si on les compare entre eux, les anticorps anti-CD38 ont démontré une activité clinique significative, seuls ou en combinaison avec diverses molécules, chez les patients atteints de MM. Ils contribueront sans aucun doute à des progrès majeurs pour la prise en charge thérapeutique des patients atteints de MM.

    GénétiqueImmunologieSémiologieLicence : https://creativecommons.org/licenses/by/4.0/
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    Anticorps monoclonaux thérapeutiques En dermatologie aussi

    Aurélie Du-Thanh, Bernard Guillot

    Therapeutic monoclonal antibodies are henceforth commonly used in the management of psoriasis but have been also used more recently in chronic spontaneous urticaria and atopic dermatitis. Three examples are developed herein: dupilumab, omalizumab and lanadelumab. The specificity of their mechanism of action results from a better understanding of the inflammatory pathways in these chronic diseases, which previously shared either the same targeted topical or systemic treatments. However, their high costs should be put into perspective with the epidemiology, the precise evaluation of the severity, the optimization of first line treatments and the long-term benefit/risk ratio.

    Biostatistiques et épidémiologiePharmacologieToxicologieLicence : https://creativecommons.org/licenses/by/4.0/
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  • Article libreArticleEn anglais

    Antimalarial drug use in general populations of tropical Africa

    Florence Gardella, Serge Assi, Fabrice Simon, Hervé Bogreau, Teunis Eggelte, Fatou Ba, Vincent Foumane, Marie-Claire Henry, Pélagie Traore Kientega, Léonardo Basco, Jean-François Trape, Richard Lalou, Maryse Martelloni, Marc Desbordes, Meïli Baragatti, Sébastien Briolant, Lionel Almeras, Bruno Pradines, Thierry Fusai, Christophe Rogier

    BackgroundThe burden of Plasmodium falciparum malaria has worsened because of the emergence of chloroquine resistance. Antimalarial drug use and drug pressure are critical factors contributing to the selection and spread of resistance. The present study explores the geographical, socio-economic and behavioural factors associated with the use of antimalarial drugs in Africa.MethodsThe presence of chloroquine (CQ), pyrimethamine (PYR) and other antimalarial drugs has been evaluated by immuno-capture and high-performance liquid chromatography in the urine samples of 3,052 children (2–9 y), randomly drawn in 2003 from the general populations at 30 sites in Senegal (10), Burkina-Faso (10) and Cameroon (10). Questionnaires have been administered to the parents of sampled children and to a random sample of households in each site. The presence of CQ in urine was analysed as dependent variable according to individual and site characteristics using a random – effect logistic regression model to take into account the interdependency of observations made within the same site.ResultsAccording to the sites, the prevalence rates of CQ and PYR ranged from 9% to 91% and from 0% to 21%, respectively. In multivariate analysis, the presence of CQ in urine was significantly associated with a history of fever during the three days preceding urine sampling (OR = 1.22, p = 0.043), socio-economic level of the population of the sites (OR = 2.74, p = 0.029), age (2–5 y = reference level; 6–9 y OR = 0.76, p = 0.002), prevalence of anti-circumsporozoite protein (CSP) antibodies (low prevalence: reference level; intermediate level OR = 2.47, p = 0.023), proportion of inhabitants who lived in another site one year before (OR = 2.53, p = 0.003), and duration to reach the nearest tarmacked road (duration less than one hour = reference level, duration equal to or more than one hour OR = 0.49, p = 0.019).ConclusionAntimalarial drug pressure varied considerably from one site to another. It was signif

    Biostatistiques et épidémiologieMicrobiologie (bactériologie, virologie)Parasitologie et mycologieLicence : This is an Open Access article distributed under the terms of the Creative Commons Attribution License (), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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  • Article libreArticleEn anglais

    Antimicrobial resistance and toxin profiles of enterotoxigenic Escherichia coli in children under five years old with diarrhoea in Ouagadougou, Burkina Faso: a sentinel surveillance study

    Alimatou Héma, Issa Tondé, Roger Kaboré, Moussa Sangaré, Mamadou Tamboura, Evariste Bako, Touwendsida Serge Bagre, Marguerite Edith Malatala Nikiema, Aminata Ouattara, M. Jahangir Hossain, Subhra Chakraborty, Mahamoudou Sanou

    BackgroundEnterotoxigenic Escherichia coli (ETEC) remains a major cause of paediatric diarrhoeal disease in sub-Saharan Africa. Increasing antimicrobial resistance (AMR) threatens the effectiveness of empirical treatment, yet data on ETEC-specific resistance patterns and toxin profiles in Burkina Faso are scarce. This study assessed the prevalence, toxin gene distribution, and antimicrobial susceptibility of ETEC among children with acute diarrhoea in Ouagadougou, Burkina Faso.MethodsA hospital-based cross-sectional sentinel surveillance study was conducted at the “Centre Hospitalier Universitaire Pédiatrique Charles De Gaulle (CHUP-CDG)”, Ouagadougou, from May 2023 to April 2024. Stool samples were collected from 383 children under five years of age presenting with acute diarrhoea. ETEC was identified by culture followed by multiplex PCR targeting eltB (LT), estA1 (STp), and estA2 (STh) genes. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disk diffusion method and interpreted according to EUCAST 2023 criteria. Multidrug resistance (MDR) was defined as non-susceptibility to at least one agent in three or more antimicrobial classes.ResultsETEC was detected in 16 of 383 children, yielding a prevalence of 4.2% (95% CI: 2.4–6.7). The highest proportion of cases occurred in children aged 12–23 months (43.8% (7/16)). LT-producing strains predominated (50.0% (8/16)), followed by STp-producing strains (43.8%), with one LT + STh isolate (6.3% (1/16)). Antimicrobial resistance was high to commonly used oral agents, including trimethoprim (93.3% (14/15)), sulfamethoxazole (73.3% (11/15)), and ampicillin (66.7% (10/15)). Resistance to third-generation cephalosporins was substantial (ceftazidime 57.1% (8/14), cefotaxime 53.3% (8/15)), and fluoroquinolone resistance reached 40.0% (6/15) for ciprofloxacin. Eleven of 15 tested isolates (73.3% (7/15)) were classified as multidrug-resistant. All isolates remained susceptible to carbapenems and cefoxitin. Wh

    Biologie cellulaire et moléculaireGénétiqueBiostatistiques et épidémiologieLicence : Open Access http://creativecommons.org/licenses/by/4.0/ This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original autho
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